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Kinetic studies are possible due to the high speed of this technique.
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UseĬonfocal technique for investigation of fluorescent living cells. With seven laser lines and the external emission filter wheel, the SDC can observe almost any fluorophore that is used for live cell microscopy. View specifications, prices, citations, reviews, and more. The Nikon Spinning Disc Confocal is an ideal microscope for long-term 3D live cell imaging. Spinning disk confocal microscopy technology, also known as multipoint (or array) scanners, is based on a light passing through pinholes on a spinning disk microscope to produce multiple excitation beams that are swept across the specimen as the disk spins. With increasing density of the confocal spots, the probability of photons emitted from a certain focal spot being detected through the pinhole of a nighboring spot rises. Compare Confocal, Confocal Laser Scanning, Spinning Disk Confocal Microscopes from leading suppliers on Biocompare. Confocal microscopy is a fluorescence-imaging technique that produces exquisitely sharp optical sections through biological specimens. Imaging many confocal spots at the same time has the disadvantage of loosing sectioning quality. While a point scanning confocal microscope images one spot at a time, a spinning disk confocal microscope illuminates about 1000 spots in parallel and detects emitted light using a camera.
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The latter is important for survival of cells during live cell imaging experiments. The difference between a point scanning confocal and a spinning disk confocal microscope is the number of image points acquired at the same time. The advatage of this approach is an increase in imaging speed and / or reducion of peak power at the illuminated spot. The difference between a point scanning confocal and a spinning disk confocal microscope is the number of image points acquired at the same time.